Harry Kane will play no part in England's friendly against Spain after being released from the squad.

The Tottenham striker was an unused substitute for Friday's 3-0 defeat of Scotland, having only just returned from a seven-week lay-off with an ankle injury.

The Football Association were thankful for Spurs' co-operation over Kane, who played 73 minutes in last weekend's north London derby and headed to St George's Park less than 24 hours later.

Interim manager Gareth Southgate was prepared to use the striker if required in the World Cup qualifier but goals from Daniel Sturridge, Adam Lallana and Gary Cahill rendered that a moot point. He will now head back to Tottenham to continue building his match fitness.

"Harry reported for us last week having not played a full game for some seven weeks but Tottenham were more than happy for him to come with us," said Southgate.

"It was never really my intention to start him in a game and I think it's important that he gets a different training programme to what we're going to follow over the next couple of days.

"I'm really grateful to Tottenham for letting us have him for the qualifier. Once we'd assessed our injury status and the fact we were fine and covered for what we'd do for this game, the sessions that we're going to do aren't what Harry needs at this time, having had such a long lay-off."

England have not called up a replacement for Kane, with Sturridge, Jamie Vardy, Marcus Rashford, Wayne Rooney and Theo Walcott all available to fill attacking roles.

The game marks the end of Southgate's temporary stint in charge of the national side, though he remains firm favourite to win a permanent deal after taking seven points from a possible nine in Group F.